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KMID : 0378019710140100125
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New Medical Journal
1971 Volume.14 No. 10 p.125 ~ p.133
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Morphological Changes of Intestinal Mucus Secretory Cells following Acute Dehydration
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Abstract
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In order to study on the morphological and histochemical changes of -mucus secretory cells in the rat intestinal mucosa following acute dehydration and during convalescence, this experiment was performed. Fifty healthy adult female rats weighing about 200gm were divided into normal, dehydrated, and convalescent groups. Dehydrated rats obtained by absolute restriction of drinking water were sacrificed at 1, 2, 3 and 5days, and convalescent ones obtained by permission of drinking water ad libitum after 5 days-restriction sacrificed at 1, 2, 3, 5 and 10 days. All of experimental rats were kept under constant room temperature and humidity.
Specimens of duodenum and colon of experimental rats were fixed in 10% neutral buffered formalin solution, embedded in paraffin wax, sectioned in 6 micra thickness, and stained with hematoxylin-eosin stain and alcian blue (pH 2.5)-periodic acid-Schiff reaction for mucous substances.
Observed results were as follows:
1. Atrophy, of the mucous membrane, severe desquamation of the surface epithelial cells, and increase of round cells in the lamina propria were seen in the duodenum and colon following acute dehydration. Severity of damage was lesser in the colonic mucosa than in the duodenal mucosa, and recovery of the colonic mucosa was faster than that of the duodenal ones in reconvalescent stage.
2. In the normal duodenal mucosa, blue or blue-purple mucus coating was seen, the goblet cells showed various tint from blue to violet according to their level in villi and, in general, alcianophilia predominated in the cells located at the tip or sides of villi, and the Brunner´s gland cells revealed alcianophilia in the luminar cytoplasm. Under acute dehydrated condition, decrease of alcianophilia in the goblet cells and Brunner´s gland cells and decrease of number of goblet cells were distinct, and during the convalescent stage, the recovery of number and mucus content of the goblet cells was prior to
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